Fascination About attune flow cytometer
Fascination About attune flow cytometer
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At the moment, the early symptoms were being dismissed as debris, though the retrospective evaluation shown shared traits Together with the problematic cells in tradition. Tracing the infection helped the lab set up added laboratory methods for screening and defense of assay-significant cell strains.
Whether or not you’re a newbie or an authority, explore our assets to advance your investigation and know-how in flow cytometry.
Merge acoustic focusing technologies with substantial-parameter Evaluation for flexible spectral unmixing and standard workflows.
The award-profitable Invitrogen Attune CytPix Flow Cytometer combines acoustic focusing by using a higher-velocity brightfield digicam for simultaneous substantial throughput flow cytometry and substantial resolution brightfield imaging.
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Furthermore, it's illuminating to find out the number of doublets slip by common singlet gates, and fantastic to validate the standing of unpredicted double-positive gatherings in my Evaluation."
Optimizing gating strategy. Even strong guide singlet gating is error-inclined and remains a subjective selection position in almost all flow cytometry assays. Imaging can be utilized to verify and alter gates to include only single cells of curiosity.
Steady image quality even at superior throughput Acoustic concentrating along with a significant-speed digital camera Incorporate to graphic these T cells continuously at minimal or superior flow premiums. Conveniently change concentration and camera settings to meet experimental prerequisites.
The Attune NxT cytometer will be the reliable workhorse instrument your lab can rely upon for regular and dependable effectiveness. The Attune CytPix product encompasses a substantial-speed brightfield digital camera and AI-driven graphic Evaluation program, with new updates together with equipment Studying equipment and an intuitive user interface, enabling enhanced information precision, accuracy, and faster insights into sample biology.
Possibly most significantly, these activities incorporate cells of clearly distinctive phenotypes which could lead on to incorrect conclusions relating to double good functions (especially in rare populations).
Seem waves help exact mobile alignment, resulting in Excellent data top quality and ~10X faster sample flow charges.
Application Observe: Automatic graphic analysis lowers consumer-to-consumer variability in flow cytometry gating approaches
Constructed-in dimensionality reduction applications greater resolution into populations. Fluorescently gated populations back-gated onto UMAP plot to verify anticipated populace resolution in lysed entire human blood. Consumer can view occasions from Each and every cluster for visual affirmation of mobile phenotypes.
No clean/no lyse leukocyte Investigation with imaging. Cells were being obtained from 24-hour-old blood by dilution in 1 mM EDTA ( Specialist UX/UI permits customers to prepare AI models for expanded apps. (A) The “Educate Product” feature provides annotation tools and click here actual-time product teaching precision.
At the center of every Attune Flow Cytometer lies our revolutionary acoustic focusing know-how, which makes use of sound waves to meticulously align cells inside the flow mobile. This modern method surpasses classic hydrodynamic methods, causing quicker processing speeds, Excellent info high-quality, and increased sample throughput.
The Attune Xenith cytometer is really a significant-parameter spectral analyzer, giving both spectral unmixing and traditional flow capabilities for extensive and versatile cell Assessment.
Agilent Worth Promise guarantees you at the least 10 years of instrument use in the day you purchase. Or else, We're going to credit rating you With all the residual worth of the program towards an upgraded model.
Listed here, we exhibit that by gating tactics working with impression-dependent quantitative parameters can distinguish interacting cells from coincident gatherings to a lot more precisely evaluate interacting cells.